Posts By: mas

A Microscopy & Analysis Conference will take place on 9th January 2013 in 3WN


📥  conference 2013

The conference aims to bring together researchers and postgraduate students from across the faculties of Science and Engineering & Design. Researchers involved in microscopy at other institutions in the South West of the UK and representatives from local companies interested in using University facilities will also attend. The conference will highlight innovative microscopy & analysis instrumentation used in nanotechnology research, and eminent speakers have been invited who use state-of-the art instruments in their own research. 

See the program below and scroll down to see a list of sponsors exhibiting at the Trade Exhibition. Scroll further to find details of speakers and their talks.

Free registration, buffet lunch and wine reception!

To register email Ursula Potter (


Microscopy & Analysis Conference Program 2013


📥  Analysis, Bioimaging, conference 2013, Electron Microscopy

Wednesday 9th January 2013

Venue: University of Bath 3 West North 2.1, 3.7 & 3.8

09:00-10:00      Registration  (coffee/tea at Trade Exhibition)

10:00-10:05      Welcome 

Jane Millar  (Pro-Vice-Chancellor of Research)

10:05-10:50      'Chemical ghosts in the Fossil Record'  

Phil Manning  (University of Manchester)  

10:50-11:35      ‘Super-resolution imaging: bacteria and beyond’

Achillefs Kapanidis  (University of Oxford) 

11:35-12:00      Break  (coffee/tea available at Trade Exhibition)

12:00-12:45      ‘The STEM multi-signal approach: learning the most from your nano- object' 

Christian Colliex  (Université Paris Sud)

12:45-14:00      Lunch Break  (Buffet lunch at Trade Exhibition)

14:00-14:45      ‘Raman microscopy for biomedical applications: from peptide nanotubes to label-free imaging of cells and tissues’

Ioan Notingher  (University of Nottingham)

14:45-15:30      ‘Electron microscopy of thin-film solar cells’

Budhika Mendis  (University of Durham)

15:30-16:00      Break  (coffee/tea at Trade Exhibition) 

16:00-16:45      Title TBA

Cinzia Casiraghi  (University of Manchester)

16:45-17:30      Deconvoluting intracellular signalling using high-content microscopy’

Jim Caunt  (University of Bath)

17:30               Wine networking reception (sponsored by Jeol UK)  poster prize awards & trade exhibits

Registration, buffet lunch & wine reception are free!

To register email Ursula Potter

Posters Welcome (contact Ursula)   

See below for more details of speakers, talks and conference sponsors.


MAS Conference 2013 - Speakers


📥  Analysis, Bioimaging, conference 2013, Electron Microscopy

Speakers at the Microscopy & Analysis Conference on 9th January 2013: 

Professor Ioan Notingher from The University of Nottingham - ‘Raman microscopy for biomedical applications: from peptide nanotubes to label-free imaging of cells and tissues’  -  Biological  Raman microscopy/spectroscopy related to the development of new optical and spectroscopic methods for studying biological materials at a nano and micro-scale.

Professor Christian Colliex  from The Université Paris Sud -  'The STEM multi-signal approach: learning the most from your nano-object’'. Development of scanning transmission electron microscopy (STEM) over the past decades, and how objects of the nanoworld (natural or artificial) can now be fully characterized individually.

Dr Phil Manning from The University of Manchester - '‘Chemical Ghosts in the Fossil Record'  New and existing techniques are used in the analysis of vertebrate locomotion, biomechanics, palaeobiology, proteomics and musculoskeletal form & function in extinct vertebrates. Successfully recovered potential breakdown products of dinosaur skin and tendon have been verified by imaging (FTIR, CL, ESEM & BSEM) and analytical (XRD, MALDI-TOF and GCMS) techniques.

Dr Cinzia Casiraghi from The University of Manchester - Title TBA - involving Raman Spectroscopy and TEM used in research with graphene, and carbon nanotubes.

Dr Achilles Kapanidis  from the University of Oxford - ‘Super-resolution imaging: bacteria and beyond’ - Ultrasensitive microscopy is used to study biological machinery involved in gene expression and regulation. Machines of gene expression are studied by observing single biomachines in real time, "in vitro" and in living cells. Our main tool is single-molecule fluorescence spectroscopy, a technique that can measure nanometre distances and study molecular interactions in real time (as "molecular movies"). The work is multidisciplinary, combining diverse disciplines such as optics, spectroscopy, biochemistry, molecular biology, computation, molecular modelling, and signal processing.

Dr Budhika Mendis  from The University of Durham - ‘Electron microscopy of thin-film solar cells’ - Silicon currently dominates solar cell manufacture despite it being a poor absorber of light. Thin-film solar cells rely on strongly light absorbing materials, such as CdTe, Cu(In,Ga)Se2 (CIGS) and Cu2ZnSnS4 (CZTS), so that the material volume is reduced and consequently the cost and production time as well. Although thin-film solar cell efficiencies have been steadily increasing over the years they are still not as commercially competitive as silicon. The microstructure plays an important role in the performance of thin-film solar cells; for example inter-diffusion at interfaces between chemically dissimilar layers during processing, electrical activity of grain boundaries, influence of secondary phase precipitates etc. In this presentation I will demonstrate how electron microscopy can provide information on a wide range of microstructural properties at high spatial resolution. Examples from chlorine activation of CdTe, secondary phases in CZTS, graded composition profiles in CIGS analysed using STEM EDX, EELS and cathodoluminescence will be presented. Time permitting I will also show how electron tomography can be used to study the role of morphology on the performance of organic solar cells.

Dr Jim Caunt from The University of Bath - Deconvoluting intracellular signalling using high-content microscopy’  - High Content Microscopy & Analysis and Confocal Microscopy are used to study how ERK and its binding partners control cell behaviour.

To register for the conference email Ursula Potter (


Raman Microscopy/Spectroscopy

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📥  Analysis, Bioimaging

Physics Equipment Shoot

(Photograph copyright University of Bath taken by Nic Delves-Broughton)

A Renishaw inVia Raman Microscope system with a full spectrum of laser sources is now running in MAS and available to book. This instrument is a high-sensitivity system with integrated research grade microscope, enabling high resolution confocal measurements. It supports multiple lasers, with automatic software switching of excitation wavelength. The instrument operates with a high through put, non-destructive technique and although lower in imaging resolution than an electron microscope it offers a more dynamic method for investigating the chemical properties of materials ranging from biological cells to semiconductor devices.

Typical investigations:

  • Chemical composition and the molecular structure of a sample.
  • Stress measurements
  • Phase imaging

With biological samples the instrument can:

  • Distinguish between cancerous, pre-cancerous and normal tissue.
  • Investigate changes in cell metabolites and protein structures.
  • Maximise the effectiveness of Raman in studying biological and biomedical materials using StreamLine™ Plus imaging.


A Perkin Elmer Frontier FTIR instrument


📥  Analysis

A Perkin Elmer Frontier FTIR instrument, now available for use in MAS, is equipped with a Diamond ATR head, a DRIFT attachment for powders, and gas and transmission cells.

The Frontier FTIR is a bench top instrument with automated range switching and mid-near-or far-IR techniques. An exceptional signal-to-noise ratio and photometric performance assures optimal spectral performance to ensure excellent sensitivity.  Typical investigations carried out with this instrument are:

  • Troubleshooting  manufacturing problems
  • Identifying product contaminants.
  • Confirming the quality of materials.

With pharmaceutical samples investigations may be:

  • To gain deeper understanding of product formulations. 
  • Rapidly screening the quality of raw materials, intermediates and formulated products.
  • Analyzing  packaging and package coatings.

See a Perkin Elmer video at:


High content imaging and analysis (HCA)

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📥  Analysis, Bioimaging

HCA combines cell biology with automated microscopy and data evaluation. For example information on a cellular event is acquired spatially and temporally and then automatically quantified. Spatially resolved instruments are typically automated microscopes, and temporal resolution requires some form of fluorescence measurement. Many HCA instruments are (fluorescence) microscopes connected to an image analysis package.

The GE Healthcare IN Cell Analyzer 2000 recently installed in MAS may be used for the detailed study of cellular mechanisms and interactions in multiple samples, for example, studying whether cell division, motility, or protein entry is affected when cells are exposed to external stimuli such as chemical inhibitors. Information generated using HCA is highly valuable in the study of complex diseases. In drug discovery research, the throughput gained using HCA makes it a valuable technology for drug target identification.

The GE Healthcare IN Cell Analyzer 2000 features include:

  • A flexible, modular, lamp based cellular and subcellular imaging system.
  • Automated imaging in fixed and live cells.
  • High performance hardware and state of the art software for cell analysis.

The  IN Cell Analyzer 2000 HCA is housed in its own room in MAS (2.14) and an analysis workstation is also available.


New Analysis Instruments

📥  Analysis, Bioimaging

Three new analysis instruments have just been installed in MAS:

  • A Perkin Elmer Frontier FTIR instrument
  • A Renishaw inVia Raman Microscope
  • A GE Healthcare IN Cell Analyzer 2000 (High Content Microscope)

Contact MAS staff for training on the instruments - this is required before users can book the instruments themselves.

See further posts for details of each instrument!


MAS Has A New Website


📥  Bioimaging, Electron Microscopy

Mas has a new website detailing all the equipment housed in the suite. Information can be found on booking instruments and how you can be trained to use them. 

There is a short video on the front page showing various instruments in use by staff and researchers.  

Have a look at the lovely examples of research images taken in MAS!

Links to the booking system and the new blog are on the front page.

Information for the 2013 Microscopy & Analysis Conference will be posted as soon as it is finalised!