Topic: Bioimaging

Microscopy & Analysis Conference 2015 - Trade Exhibition

📥  Analysis, Bioimaging, Conference 2015, Electron Microscopy

The following companies will be exhibiting their products at the conference on 6th May 2015:

Abcam  -  Baker Ruskinn  Buehler UK  - Bruker - CNTech  -  EM RESOLUTIONS - Hitachi  JEOL  -  Leica  -  Nikon  -  Olympus - Oxford Instruments - Quorum Technologies  -  Renishaw  -  Scanwel -  TESCAN-UK  - Thermo Scientific  - Zeiss  -  Tebu-Bio

Conference Programme 2015
To sign up email U.J.Potter@bath.ac.uk

 

Conference 2015

📥  Analysis, Bioimaging, Conference 2015, Electron Microscopy

Microscopy & Analysis Conference May 6th 2015

Conference Programme 2015
(To sign up email U.J.Potter@bath.ac.uk)

Speakers:

Professor Paul Midgley FRS
(Professor of Materials Science and Director of the Electron Microscopy Facility at the University of Cambridge)

Paul’s research involves electron tomography and 3D reconstruction of nano-structures. By recording a tilt series of scanning transmission electron micrographs using high angle scattering or energy filtered images using fixed beam methods it is possible to reconstruct the 3-dimensional structure and composition of nanoscale materials. Field emission TEM, off-axis and in-line (Fresnel) electron holography is being used to study a variety of material properties: Electric Fields in biased and non-biased p-n junctions, metal oxide grain boundaries and ferroelectrics; Magnetic Fields in magnetic nanowires, thin film devices and small single domain particles.
See: http://onlinelibrary.wiley.com/doi/10.1002/anie.201400625/abstract
Conference talk title:   ‘Multi-Dimensional Electron Microscopy’

Dr Anne-Cecile Reymann
Is part of the Stephen Grill Lab at Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. Anne-Cecile, Stephen Grill and Jennifer Lippencott-Schwartz are co-authors on the recent Science article “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution”
See the stunning images and videos at: http://www.sciencemag.org/content/346/6208/1257998.full

Anne-Cecile will speak about Light Sheet Microscopy and her current research.
Conference talk title: “Light sheet versus spinning disk confocal microscopy for nematic order parameter quantification as a measure of the orientation of the actin filaments”

Professor Martin Lee
(School of Geographical and Earth Sciences at the University of Glasgow)

Martin will speak about the application of high-resolution electron and ion beam techniques, also electron backscatter diffraction (EBSD) to understanding the mechanisms and rates of mineral crystallization and dissolution in a wide range of Earth and extraterrestrial materials.
See: http://web2.ges.gla.ac.uk/~mlee/fpnano2.html and http://web2.ges.gla.ac.uk/~mlee/worms.html
Conference talk title: “SEM-EBSD/TEM techniques for investigating nanoscale feldspar weathering, building stone decay, sandstone diagenesis & invertebrate biomineralization.”

Dr Sue Vaughan
(Dept of Biological and Medical Sciences, Oxford Brookes University)

One of the major drawbacks in transmission electron microscopy has been the production of three-dimensional views of cells and tissues. Currently, there is no one suitable 3D microscopy technique that answers all questions and serial block face scanning electron microscopy (SEM) fills the gap between 3D imaging using high-end fluorescence microscopy and the high resolution offered by electron tomography.
See:   http://link.springer.com/article/10.1007/s00709-013-0580-1
Conference talk title: "Serial block face scanning electron microscopy - the future of cell ultrastructure imaging"

Dr Fernando Castro
(National Physical Laboratory, Teddington)

Fernando's research aims to develop a systematic, microscopic understanding of the relationship between nanostructure and optoelectronic properties of organic semiconductors and to correlate it with the device functionality and performance. Specific objectives include: (1) to determine three-dimensional thin film structures of organic blends, (2) to examine molecular orientation in these organic thin films, (3) to correlate thin film structure and molecular orientation to their optoelectronic properties of organic blend PV devices, and (4) to develop advanced nanoscale Raman spectroscopy.
See: http://iopscience.iop.org/0957-4484/21/49/492001/
Conference talk title: “Organic photovoltaics: principles and techniques for nanometre scale characterization using AFM, Raman, PL and depth profiling"

Dr Tom Scott
Executive Director of the Bristol-Oxford Nuclear Research Centre and Director of the Interface Analysis Centre, a specialist surface and materials science research centre, within the School of Physics at the University of Bristol. Tom is also a lecturer in Earth Sciences, specialising in research relating to the transport and clean-up of radioactive materials in the environment.
Conference talk title: “Uses of FIB/SEM in research at the Interface Analysis Centre at University of Bristol”

Dr Amanda MacKenzie
(Department of Pharmacy & Pharmacology, University of Bath).

Amanda has been awarded a BBSRC research grant for equipment consisting of a dual hypoxic workstation (comprising two chambers with individually controlled atmospheres and an integral fluorescence microscope), a spin resonance instrument and a microplate reader. The instruments will form a unique facility at the University of Bath open to researchers from GW4 institutions.

Conference talk: "A Facility for Advanced Imaging and Analysis under Hypoxic Conditions for Bath and GW4 Partners"

Conference Programme 2015

 

 

 

Introduction to MAS Facilities

📥  Analysis, Bioimaging, Electron Microscopy

Wednesday 3rd December 2014   14:15-16:00 - 6E 2.2
Presentations by MAS staff outlining the microscopy and analytical facilities available to University of Bath researchers, postgraduate students and undergraduate project students in the Microscopy & Analysis Suite.

Topics including:

  • Introduction to MAS - new booking system, training, conference.
  • Bioimaging facilities - confocal microscopy, flow cytometry, and high content microscopy.
  • SEM, FESEM, TEM and X-ray analysis with hard/soft materials, biological materials, ploymers and gels.
  • Raman Microscopy and FTIR with solid samples, powders and liquids.

Email Ursula Potter (u.j.potter@bath.ac.uk) to sign up.

 

Microscopy & Analysis Conference Program 2013

  

📥  Analysis, Bioimaging, conference 2013, Electron Microscopy

Wednesday 9th January 2013

Venue: University of Bath 3 West North 2.1, 3.7 & 3.8

09:00-10:00      Registration  (coffee/tea at Trade Exhibition)

10:00-10:05      Welcome 

Jane Millar  (Pro-Vice-Chancellor of Research)

10:05-10:50      'Chemical ghosts in the Fossil Record'  

Phil Manning  (University of Manchester)  

10:50-11:35      ‘Super-resolution imaging: bacteria and beyond’

Achillefs Kapanidis  (University of Oxford) 

11:35-12:00      Break  (coffee/tea available at Trade Exhibition)

12:00-12:45      ‘The STEM multi-signal approach: learning the most from your nano- object' 

Christian Colliex  (Université Paris Sud)

12:45-14:00      Lunch Break  (Buffet lunch at Trade Exhibition)

14:00-14:45      ‘Raman microscopy for biomedical applications: from peptide nanotubes to label-free imaging of cells and tissues’

Ioan Notingher  (University of Nottingham)

14:45-15:30      ‘Electron microscopy of thin-film solar cells’

Budhika Mendis  (University of Durham)

15:30-16:00      Break  (coffee/tea at Trade Exhibition) 

16:00-16:45      Title TBA

Cinzia Casiraghi  (University of Manchester)

16:45-17:30      Deconvoluting intracellular signalling using high-content microscopy’

Jim Caunt  (University of Bath)

17:30               Wine networking reception (sponsored by Jeol UK)  poster prize awards & trade exhibits

Registration, buffet lunch & wine reception are free!

To register email Ursula Potter U.J.Potter@bath.ac.uk

Posters Welcome (contact Ursula)   

See below for more details of speakers, talks and conference sponsors.

 

MAS Conference 2013 - Speakers

  

📥  Analysis, Bioimaging, conference 2013, Electron Microscopy

Speakers at the Microscopy & Analysis Conference on 9th January 2013: 

Professor Ioan Notingher from The University of Nottingham - ‘Raman microscopy for biomedical applications: from peptide nanotubes to label-free imaging of cells and tissues’  -  Biological  Raman microscopy/spectroscopy related to the development of new optical and spectroscopic methods for studying biological materials at a nano and micro-scale.

Professor Christian Colliex  from The Université Paris Sud -  'The STEM multi-signal approach: learning the most from your nano-object’'. Development of scanning transmission electron microscopy (STEM) over the past decades, and how objects of the nanoworld (natural or artificial) can now be fully characterized individually.

Dr Phil Manning from The University of Manchester - '‘Chemical Ghosts in the Fossil Record'  New and existing techniques are used in the analysis of vertebrate locomotion, biomechanics, palaeobiology, proteomics and musculoskeletal form & function in extinct vertebrates. Successfully recovered potential breakdown products of dinosaur skin and tendon have been verified by imaging (FTIR, CL, ESEM & BSEM) and analytical (XRD, MALDI-TOF and GCMS) techniques.

Dr Cinzia Casiraghi from The University of Manchester - Title TBA - involving Raman Spectroscopy and TEM used in research with graphene, and carbon nanotubes.

Dr Achilles Kapanidis  from the University of Oxford - ‘Super-resolution imaging: bacteria and beyond’ - Ultrasensitive microscopy is used to study biological machinery involved in gene expression and regulation. Machines of gene expression are studied by observing single biomachines in real time, "in vitro" and in living cells. Our main tool is single-molecule fluorescence spectroscopy, a technique that can measure nanometre distances and study molecular interactions in real time (as "molecular movies"). The work is multidisciplinary, combining diverse disciplines such as optics, spectroscopy, biochemistry, molecular biology, computation, molecular modelling, and signal processing.

Dr Budhika Mendis  from The University of Durham - ‘Electron microscopy of thin-film solar cells’ - Silicon currently dominates solar cell manufacture despite it being a poor absorber of light. Thin-film solar cells rely on strongly light absorbing materials, such as CdTe, Cu(In,Ga)Se2 (CIGS) and Cu2ZnSnS4 (CZTS), so that the material volume is reduced and consequently the cost and production time as well. Although thin-film solar cell efficiencies have been steadily increasing over the years they are still not as commercially competitive as silicon. The microstructure plays an important role in the performance of thin-film solar cells; for example inter-diffusion at interfaces between chemically dissimilar layers during processing, electrical activity of grain boundaries, influence of secondary phase precipitates etc. In this presentation I will demonstrate how electron microscopy can provide information on a wide range of microstructural properties at high spatial resolution. Examples from chlorine activation of CdTe, secondary phases in CZTS, graded composition profiles in CIGS analysed using STEM EDX, EELS and cathodoluminescence will be presented. Time permitting I will also show how electron tomography can be used to study the role of morphology on the performance of organic solar cells.

Dr Jim Caunt from The University of Bath - Deconvoluting intracellular signalling using high-content microscopy’  - High Content Microscopy & Analysis and Confocal Microscopy are used to study how ERK and its binding partners control cell behaviour.

To register for the conference email Ursula Potter (U.J.Potter@bath.ac.uk).

 

Raman Microscopy/Spectroscopy

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📥  Analysis, Bioimaging

Physics Equipment Shoot

(Photograph copyright University of Bath taken by Nic Delves-Broughton)

A Renishaw inVia Raman Microscope system with a full spectrum of laser sources is now running in MAS and available to book. This instrument is a high-sensitivity system with integrated research grade microscope, enabling high resolution confocal measurements. It supports multiple lasers, with automatic software switching of excitation wavelength. The instrument operates with a high through put, non-destructive technique and although lower in imaging resolution than an electron microscope it offers a more dynamic method for investigating the chemical properties of materials ranging from biological cells to semiconductor devices.

Typical investigations:

  • Chemical composition and the molecular structure of a sample.
  • Stress measurements
  • Phase imaging

With biological samples the instrument can:

  • Distinguish between cancerous, pre-cancerous and normal tissue.
  • Investigate changes in cell metabolites and protein structures.
  • Maximise the effectiveness of Raman in studying biological and biomedical materials using StreamLine™ Plus imaging.

 

High content imaging and analysis (HCA)

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📥  Analysis, Bioimaging

HCA combines cell biology with automated microscopy and data evaluation. For example information on a cellular event is acquired spatially and temporally and then automatically quantified. Spatially resolved instruments are typically automated microscopes, and temporal resolution requires some form of fluorescence measurement. Many HCA instruments are (fluorescence) microscopes connected to an image analysis package.

The GE Healthcare IN Cell Analyzer 2000 recently installed in MAS may be used for the detailed study of cellular mechanisms and interactions in multiple samples, for example, studying whether cell division, motility, or protein entry is affected when cells are exposed to external stimuli such as chemical inhibitors. Information generated using HCA is highly valuable in the study of complex diseases. In drug discovery research, the throughput gained using HCA makes it a valuable technology for drug target identification.

The GE Healthcare IN Cell Analyzer 2000 features include:

  • A flexible, modular, lamp based cellular and subcellular imaging system.
  • Automated imaging in fixed and live cells.
  • High performance hardware and state of the art software for cell analysis.

The  IN Cell Analyzer 2000 HCA is housed in its own room in MAS (2.14) and an analysis workstation is also available.

 

New Analysis Instruments

📥  Analysis, Bioimaging

Three new analysis instruments have just been installed in MAS:

  • A Perkin Elmer Frontier FTIR instrument
  • A Renishaw inVia Raman Microscope
  • A GE Healthcare IN Cell Analyzer 2000 (High Content Microscope)

Contact MAS staff for training on the instruments - this is required before users can book the instruments themselves.

See further posts for details of each instrument!

 

MAS Has A New Website

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📥  Bioimaging, Electron Microscopy

Mas has a new website detailing all the equipment housed in the suite. Information can be found on booking instruments and how you can be trained to use them. 

There is a short video on the front page showing various instruments in use by staff and researchers.  

Have a look at the lovely examples of research images taken in MAS!

Links to the booking system and the new blog are on the front page.

http://www.bath.ac.uk/mas/

Information for the 2013 Microscopy & Analysis Conference will be posted as soon as it is finalised!