Tagged: Analysis

Raman Microscopy/Spectroscopy

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📥  Analysis, Bioimaging

Physics Equipment Shoot

(Photograph copyright University of Bath taken by Nic Delves-Broughton)

A Renishaw inVia Raman Microscope system with a full spectrum of laser sources is now running in MAS and available to book. This instrument is a high-sensitivity system with integrated research grade microscope, enabling high resolution confocal measurements. It supports multiple lasers, with automatic software switching of excitation wavelength. The instrument operates with a high through put, non-destructive technique and although lower in imaging resolution than an electron microscope it offers a more dynamic method for investigating the chemical properties of materials ranging from biological cells to semiconductor devices.

Typical investigations:

  • Chemical composition and the molecular structure of a sample.
  • Stress measurements
  • Phase imaging

With biological samples the instrument can:

  • Distinguish between cancerous, pre-cancerous and normal tissue.
  • Investigate changes in cell metabolites and protein structures.
  • Maximise the effectiveness of Raman in studying biological and biomedical materials using StreamLine™ Plus imaging.


A Perkin Elmer Frontier FTIR instrument


📥  Analysis

A Perkin Elmer Frontier FTIR instrument, now available for use in MAS, is equipped with a Diamond ATR head, a DRIFT attachment for powders, and gas and transmission cells.

The Frontier FTIR is a bench top instrument with automated range switching and mid-near-or far-IR techniques. An exceptional signal-to-noise ratio and photometric performance assures optimal spectral performance to ensure excellent sensitivity.  Typical investigations carried out with this instrument are:

  • Troubleshooting  manufacturing problems
  • Identifying product contaminants.
  • Confirming the quality of materials.

With pharmaceutical samples investigations may be:

  • To gain deeper understanding of product formulations. 
  • Rapidly screening the quality of raw materials, intermediates and formulated products.
  • Analyzing  packaging and package coatings.

See a Perkin Elmer video at:


High content imaging and analysis (HCA)

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📥  Analysis, Bioimaging

HCA combines cell biology with automated microscopy and data evaluation. For example information on a cellular event is acquired spatially and temporally and then automatically quantified. Spatially resolved instruments are typically automated microscopes, and temporal resolution requires some form of fluorescence measurement. Many HCA instruments are (fluorescence) microscopes connected to an image analysis package.

The GE Healthcare IN Cell Analyzer 2000 recently installed in MAS may be used for the detailed study of cellular mechanisms and interactions in multiple samples, for example, studying whether cell division, motility, or protein entry is affected when cells are exposed to external stimuli such as chemical inhibitors. Information generated using HCA is highly valuable in the study of complex diseases. In drug discovery research, the throughput gained using HCA makes it a valuable technology for drug target identification.

The GE Healthcare IN Cell Analyzer 2000 features include:

  • A flexible, modular, lamp based cellular and subcellular imaging system.
  • Automated imaging in fixed and live cells.
  • High performance hardware and state of the art software for cell analysis.

The  IN Cell Analyzer 2000 HCA is housed in its own room in MAS (2.14) and an analysis workstation is also available.